Journal: BMC Neuroscience

Article Title: Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson's disease

doi: 10.1186/1471-2202-10-109

Figure Lengend Snippet: Whole-mount confocal immunofluorescence analysis of TH+ neurons in the DMC of Ddc-Gal4>DJ-1A RNAi flies . Whole-mount brain samples of 25-day old Ddc-Gal4>UAS-DJ-1A RNAi ( A ) or control Ddc-Gal4>UAS-GFP ( B ) flies were immunostained with anti-TH antibody and TH-positive immunofluorescence signals were detected by confocal microscopy. Representative confocal images of the TH+ neurons in the DMC are shown. Asterisks mark the identifiable TH+ neurons. Note that not only was there a reduction of TH+ neurons in Ddc-Gal4>UAS-DJ-1A RNAi flies compared to the control, the intensity of TH immunoreactivity was also reduced in the remaining neurons.

Article Snippet: Anti-TH polyclonal antibody (1:100, Pel Freez Biologicals) served as primary antibody.

Techniques: Immunofluorescence, Confocal Microscopy

Journal: BMC Neuroscience

Article Title: Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson's disease

doi: 10.1186/1471-2202-10-109

Figure Lengend Snippet: Immunohistochemical analysis showing age-dependent loss of TH+ neurons in the DMC of Ddc-Gal4>DJ-1A RNAi flies . A-D : Frontal paraffin sections of adult fly brain were reacted with anti-TH antibody and detected with the peroxidase/DAB staining method. Representative sections containing DA neurons in the DMC were shown for each genotype. A, B : Ddc-Gal4>DJ-1A RNAi flies; C, D: Ddc-Gal4/+ flies. A, C : TH-immunostaining of 1-day-old flies; B, D : TH-immunostaining of 25-day-old flies. E : Statistical analysis of the number of DNs within the DMC of Ddc-Gal4/+ , Ddc-Gal4>DJ-1A RNAi , and Ddc-Gal4>DJ-1A RNAi+hDJ-1 genotyped flies. Flies were fed with wet yeast paste. Between 8-25 fly heads for each genotype and age were evaluated. Columns depict mean with standard deviation of the mean (SD). ***: P < 0.0001 in ANOVA with Dunnett's post test.

Article Snippet: Anti-TH polyclonal antibody (1:100, Pel Freez Biologicals) served as primary antibody.

Techniques: Immunohistochemical staining, Staining, Immunostaining, Standard Deviation

Journal: BMC Neuroscience

Article Title: Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson's disease

doi: 10.1186/1471-2202-10-109

Figure Lengend Snippet: Immunohistochemical analysis of TH+ DA neurons in the DMC to show the effects of various drug treatments . Frontal paraffin sections of adult fly brain were stained with anti-TH antibody. Representative sections containing DA neurons in the DMC were shown for each genotype. 25-day-old female flies were analyzed. A-F : Ddc-Gal4>DJ-1A RNAi flies. A : yeast fed no-drug control, B : 20 μg/ml celastrol-treated, C : 50 mg/ml minocycline-treated, D : 5 μg/ml celastrol + 25 mg/ml minocycline-treated, E : 30 mg/ml NBQX-treated, F : 100 mg/ml coQ10-treated. G, H : Ddc-Gal4/+ flies. G : yeast fed no-drug control, H : 30 mg/ml NBQX-treated.

Article Snippet: Anti-TH polyclonal antibody (1:100, Pel Freez Biologicals) served as primary antibody.

Techniques: Immunohistochemical staining, Staining